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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 19, No. 10, 2021, pp. 873-880
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Bioline Code: rm21091
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 19, No. 10, 2021, pp. 873-880
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Association of the single nucleotide polymorphism C1858T of the PTPN22 gene with unexplained recurrent pregnancy loss: A case-control study
Khanbarari, Fateme; Ghasemi, Nasrin; Vakili, Mahmood & Samadi, Morteza
Abstract
Background: Lymphoid-tyrosine-phosphatase which is encoded by the protein
tyrosine phosphatase non-receptor 22 (PTPN22) gene plays a pivotal role in the
regulation of immune responses by dephosphorylating several signaling intermediates
of immune cells.
Objective: Since a balanced immune response has been shown to be important during
pregnancy, the purpose of this research was to compare the frequency of the PTPN22
C1858T polymorphism in women with unexplained recurrent pregnancy loss (URPL) vs.
in a control group for the first time.
Materials and Methods: Genomic DNA from 200 individuals with URPL and 200
individuals without URPL (the control group) at the infertility center in Yazd, Iran was
isolated using the salting-out method. The PTPN22 C1858T polymorphism of the
two groups was analyzed using polymerase chain reaction-restriction fragment length
polymorphism. Genotype frequencies in the women with URPL and the fertile control
group were compared using the Chi-square test.
Results: There were significant differences in the frequency of the PTPN22 1858T
polymorphism in the URPL individuals vs. the healthy controls, i.e. 32.0% and 21.5%,
respectively (p = 0.01).
Conclusion: Our findings suggest that the PTPN22 1858T polymorphism could
play a role in recurrent pregnancy loss. Therefore, genotyping of the mentioned
polymorphism can help clinicians to predict the probable risk of URPL.
Keywords
Recurrent pregnancy loss; PTPN22 protein; Single nucleotide polymorphism.
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