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Effects of melatonin and human follicular fluid supplementation of in vitro maturation medium on mouse vitrified germinal vesicle oocytes: A laboratory study
Doroudi, Razieh; Changizi, Zohre & Nematollahi-Mahani, Seyed Noureddin
Abstract
Background: Vitrification as the most efficient method of cryopreservation, enables
successful storage of oocytes for couples who undergo specific procedures including
surgery and chemotherapy. However, the efficacy of in vitro maturation (IVM) methods
with vitrified germinal vesicle (GV) oocytes could be improved.
Objective: As melatonin and follicular fluid (FF) might enhance IVM conditions, we
used these supplements to assess the maturation rate of vitrified GV oocytes and their
artificial fertilization rate.
Materials and Methods: Four hundred mouse GV oocytes were harvested, vitrified,
and assigned into control (C-Vit-GV) and treatment groups of melatonin (M-Vit-GV),
human follicular fluid (HFF-Vit-GV), and a combination (M + HFF-Vit-GV). A non-vitrified
group of GV oocytes (non-Vit-GV) and a group of in vivo matured metaphase
II (Vivo-MII) oocytes served as control groups to evaluate the vitrification and IVM
conditions, respectively. Maturation of GV oocytes to MII and further development to
two-cell-stage embryos were determined in the different groups.
Results: Development to two-cell embryos was comparable between the Vivo-MII and
non-Vit-GV groups. IVM and in vitro fertilization (IVF) results in the non-Vit-GV group
were also comparable with the C-Vit-GV oocytes. In addition, the IVM and IVF outcomes
were similar across the different treatment groups including the M-Vit-GV, HFF-Vit-GV,
M + HFF-Vit-GV, and C-Vit-GV oocytes.
Conclusion: Employing an appropriate technique of vitrification followed by suitable
IVM conditions can lead to reasonable IVF outcomes which may not benefit from extra
supplementations. However, whether utilizing other supplementation formulas could
improve the outcome requires further investigation.
Keywords
Vitrification; Germinal vesicle; In vitro oocyte maturation; Melatonin; Follicular fluid.
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