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The effects of supplemented sericin on in vitro maturation and preimplantation development of mouse embryos: An experimental study
Banafshi, Omid; Nasseri, sSherko; Farhadi, Leila; Alasvand, Masoud; Khadem-Erfan, Mohammad Bagher; Hosseini, Javad; Miraki, Saber & Fathi, Fardin
Abstract
Background: Mouse embryo culture condition is an essential part of transgenic,
reproductive and developmental biology laboratories. Mouse embryonic culture media
may have a high risk of serum contamination with pathogens.
Objective: To investigate the effect of sericin as an embryo culture medium supplement
on in vitro maturation (IVM), in vitro fertilization (IVF), and development of the
preimplantation embryo in mice.
Materials and Methods: The effects of sericin at three concentrations (subgroups) of
0.1%, 0.5%, and 1% as a medium supplement on IVM, IVF, and in vitro development
of mouse embryos were separately investigated and compared with a sericin-free
(control) group. The cumulative effect of the three concentrations was evaluated for
IVM + in vitro development and IVF + in vitro development as follow-up groups.
Results: In the IVM group, compared to the control group, the number of
oocysts reaching the MII stage was significantly higher when 1% sericin was used
(161/208 = 77.4%). No significant
results were observed in the IVF and in vitro
development groups with different concentrations of sericin compared to the control
group. Among the follow-up groups, in the IVM + in vitro development group, the
number of oocytes was higher after passing the IVM and IVF and reaching the
blastocysts stage when 1% sericin was used, compared with other sericin subgroups. A
significant difference was also noted when compared with the control group (p = 0.048).
The IVF + in vitro development study group, on the other hand, did not show any
significant relationship.
Conclusion: It can be concluded that 1% sericin can be used as a supplement in mouse
embryo cultures to improve the IVM rate. Also, based on the findings, sericin appears
to be an effective supplement which can have a positive effect on the development of
embryos derived from IVM.
Keywords
Sericin; In vitro maturation; In vitro fertilization; Preimplantation embryo; Culture medium; Mice.
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