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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 19, No. 11, 2021, pp. 987-996
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Bioline Code: rm21102
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 19, No. 11, 2021, pp. 987-996
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The reaggregation of normal granulosa-cumulus cells and mouse oocytes with polycystic ovarian syndrome in vitro: An experimental study
Moradi, Amaneh; Ghasemian, Fatemeh & Mashayekhi, Farhad
Abstract
Background: The dialogue between oocytes and their surrounding cells plays a major
role in the progress of oocyte meiosis and their developmental potential.
Objective: This study aimed to evaluate the effect of co-culture of normal granulosacumulus
cells (GCCs) with oocytes from polycystic ovarian syndrome (PCOS) mice.
Materials and Methods: Normal GCCs were collected from 10 virgin adult Naval
Medical Research Institute female mice (30-35 gr, 7-8 wk old), and were cultured in
an alpha-minimum essential medium supplemented with 5% fetal calf serum for 24-48
hr (1×106 cells/well). Then, germinal-vesicle oocytes from PCOS mice were cultured
in the presence of cultured normal GCCs (experimental group) and without GCCs
(control group). The maturation rate and quality of the PCOS oocytes were examined
by evaluating TFAM and Cx43 gene expression (real-time PCR) and the connection
among PCOS oocytes and normal GCCs after 24 hr of culture.
Results: The co-culture of normal GCCs and PCOS oocytes in the experimental group
led to the formation of a complex called a PCOS oocyte-normal GCCs complex. The
maturation rate of these complexes was significantly increased compared to that of
the control group (p ≤ 0.001). A significant difference was also found in the expression
of Cx43 (p ≤ 0.001) and TFAM (p < 0.05) genes in the experimental group compared
with the control group. The connection between PCOS oocytes and normal GCCs was
observed in the scanning electron microscope images.
Conclusion: Co-culture with normal GCCs improves the capacity of PCOS oocytes to
enter meiosis, which may result in the promotion of assisted reproduction techniques.
Keywords
PCOS; Co-culture; Granulosa-cumulus cells; IVM; Cx43.
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