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The effect of cult-active medium on pregnancy outcomes after intracytoplasmic sperm injection in azoospermic men: A case-control study
Asa, Elham; Janatifar, Rahil; Sahraei, Seyedeh Saeideh; Verdi, Atefeh & Kalhor, Naser
Abstract
Background: Failed oocyte activation following intracytoplasmic sperm injection (ICSI)
as a result of calcium deficiency is a major challenge.
Objective: We compared the effect of cult-active medium (CAM) on ICSI outcomes in
obstructive azoospermia cases.
Materials and Methods: The present study was conducted with 152 ICSI cases,
classified into CAM and control groups. The injected oocytes in the control group were
cultured in the cleavage medium, while in the artificial oocyte activation group, oocytes
were chemically activated through exposure to 200 μL of CAM for 15 min. Fertilization
and cleavage rates, quality of embryos, and biochemical pregnancy and live birth rates
were assessed in both groups.
Results: There were significant differences between the groups in terms of fertilization
and cleavage rates after using the CAM in the percutaneous epididymal sperm
aspiration (PESA) subgroup (p = 0.05, p ≤ 0.001) and in the testicular sperm extraction
subgroup (p = 0.02, p = 0.04), compared to their control groups. Also, the pregnancy
rate was significantly higher in the PESA-CAM subgroup (p = 0.03). The PESA-CAM
subgroup demonstrated a significant difference in embryo quality after ICSI (p = 0.04).
Unsuccessful embryo transfer and abortion were lower in both subgroups compared
to the control groups, but this difference was not significant. Surprisingly, live birth rate
was higher in the PESA-CAM subgroup (p = 0.03).
Conclusion: CAM treatment could improve fertilization and cleavage rates in
obstructive azoospermia participants. It had a significant effect on embryo quality, and
pregnancy and live birth rates in PESA cases.
Keywords
Calcium ionophore; Obstructive azoospermia; Fertilization; ICSI.
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