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Basella alba , Grewia asiatica , Solanum nigrum and Ficus carica fruit extracts ameliorate the testicular histopathology induced by CCl4 exposure in albino mice: An experimental study
Ahmad, Syeda Nadia; Ahmad, Khawaja Raees; Abdullah, Usma; Malik, Fiza; Ali, Rabiyah; Amir-ud-din, Umara; Kanwal, Muhammad Ali & Inayat, Iram
Abstract
Background: Carbon tetrachloride (CCl4) is extensively used in various industries and
induces oxidative stress in humans. Unfortunately, it is one of the neglected potent
male reproductive toxicants.
Objective: The present research reports the testicular histopathology of CCl4 and
ameliorations by four medicinal fruit pulp extracts (FPEs) in mice.
Materials and Methods: Sixty male albino mice were divided into six groups (10/group)
as per the following: 1. Vehicle control (Vc); 2. CCl4 (C); 3-6. CCl4+Basella alba (CBa),
CCl4+Solanum nigrum (CSn), CCl4+Ficus carica (CFc) and CCl4+Grewia asiatica (CGa).
Except for the control group, CCl4 (0.1 mL of 0.2 mL kg−1) was given to the animals in
corn oil. The four plant extracts (0.1 mL each) were respectively given to the relevant
FPE group animals for the next five consecutive days, while the animals in the Vc and
CCl4 groups received water instead of FPE.
Results: The CCl4 exposure led to various histometric and histological alterations
(loss of interstitial tissue and various dislodged tailless spermatids with enlarged
heads) that were recovered in all except Solanum nigrum FPE mice post-treatment.
The micrometric data of testicular sections also indicated significant decline in the
number of spermatogonia, while the cross-sectional area of the sperm heads remained
significantly higher in the CSn and C groups.
Conclusion: Except for Solanum nigrum, the three FPEs, especially Ficus carica,
showed rehabilitative properties against CCl4 exposure-related modifications in
testicular histopathologies.
Keywords
Basella alba; Grewia asiatica; Solanum nigrum; Ficus carica; testis.
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