search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Iranian Journal of Environmental Health, Science and Engineering
Iranian Association of Environmental Health (IAEH)
ISSN: p-ISSN: 1735-1979
Vol. 2, No. 3, 2005, pp. 207-211
Bioline Code: se05030
Full paper language: English
Document type: Research Article
Document available free of charge

Iranian Journal of Environmental Health, Science and Engineering, Vol. 2, No. 3, 2005, pp. 207-211

 en STUDY OF BACTERIAL RESISTANCE TO ORGANOPHOSPHOROUS PESTICIDES IN IRAN
A. Nazarian and M. Mousawi

Abstract

The broadness application of organophosphorus compounds has abounded the number of its polluted areas. Bioremediation has widely focused on insitu bacterial degradation of organophosphorus residues in the world. Therefore, in this research six numbers of samples from two different sources, soil and water randomly were isolated using different organophosphorus pesticides containing mineral solution without supplementation. More than 100 isolated strains were selected according to their simultaneous optimal growth on mineral medium with organophosphorus and Mac Conkey's agar. More than 50 percent of them were lost above resistance. The resistant strains were identified by two methods, the biochemical convention and API 20E procedure with positive agreement. The identified strains belonged to Pseudomonas   check for this species in other resources and Flavobacterium   check for this species in other resources species. The maximum tolerant concentrations of different organophosphorus pesticides by these resistant strains were 2.5, 4 and 8 g/L of guthion, methyl parathion and Dimethoate, respectively. The resistance to these pesticides due to organ phosphorous degrading plasmids had the ability to express hydrolytic enzymes. Resistant bacteria lost these plasmids by acridin orange and could translocate to sensitive strains. Thus, certain environmental bacteria could be used as protection tools against antinerve agents.

Keywords
Bacterial resistance, bioremediation, organophosphorous pestisides, antinerve agents

 
© Copyright 2005 - Tehran University of Medical Sciences Publications
Alternative site location: http://diglib.tums.ac.ir/pub/

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2019, Site last up-dated on 10-Jul-2019.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil