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Iranian Journal of Environmental Health, Science and Engineering
Iranian Association of Environmental Health (IAEH)
ISSN: 1735-1979
Vol. 4, No. 2, 2007, pp. 93-98
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Bioline Code: se07014
Full paper language: English
Document type: Research Article
Document available free of charge
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Iranian Journal of Environmental Health, Science and Engineering, Vol. 4, No. 2, 2007, pp. 93-98
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EXTRACTION OF ASTAXANTHIN ESTERS FROM SHRIMP WASTE BY CHEMICAL AND MICROBIAL METHODS
Khanafari, A.; Saberi, A.; Azar, M.; Vosooghi, Gh.; Jamili, Sh. & Sabbaghzadeh, B.
Abstract
The carotenoid pigments specifically astaxanthin has many significant applications in food,
pharmaceutical and cosmetic industries. The goal of this research was the extraction of Astaxanthin
from a certain Persian Gulf shrimp species waste (Penaeus semisulcatus), purification and identification
of the pigment by chemical and microbial methods. Microbial fermentation was obtained by
inoculation of two Lactobacillus species Lb. plantarum and Lb. acidophilus in the medium culture
containing shrimp waste powder by the intervention of lactose sugar, yeast extract, the composition of
Both and the coolage (-20°C). The carotenoids were extracted by an organic solvent system. After
purification of astaxanthin with the thin layer chromatography method by spectrophotometer, NMR and
IR analysis the presence of astaxanthin esters was recognized in this specific species of Persian Gulf
shrimp. Results obtained from this study showed that the coolage at –20 °C not only does not have an
amplifying effect on the production of astaxanthin but also slightly reduces this effect. Also the effect
of intervention of lactose sugar showed more effectiveness in producing astaxanthin than yeast extract
or more than with the presence of both. The results also indicated that there is not much difference in
the ability of producing the pigment by comparing both Lb. plantarum and Lb. acidophillus. Also
results showed the microbial method of extraction of astaxanthin is more effective than chemical
method. The pigment extracted from certain amount of shrimp powder, 23.128 mg/g, was calculated.
Keywords
Astaxanthin, extraction, shrimp waste, Lactobacillus
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