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Iranian Journal of Environmental Health, Science and Engineering
Iranian Association of Environmental Health (IAEH)
ISSN: p-ISSN: 1735-1979
Vol. 5, No. 4, 2008, pp. 251-256
Bioline Code: se08041
Full paper language: English
Document type: Research Article
Document available free of charge

Iranian Journal of Environmental Health, Science and Engineering, Vol. 5, No. 4, 2008, pp. 251-256

 en Application Of Metal Resistant Bacteria By Mutational Enhancment Technique For Bioremediation Of Copper And zinc From Industrial Wastes
Shakibaie, M. R.; Khosravan, A.; Frahmand, A. & Zare, S.

Abstract

In this research, using mutation in the metal resistant bacteria, the bioremediation of the copper and zinc from copper factory effluents was investigated. Wastewater effluents from flocculation and rolling mill sections of a factory in the city of Kerman were collected and used for further experiments. 20 strains of Pseudomonas   check for this species in other resources spp. were isolated from soil and effluents surrounding factory and identified by microbiological methods. Minimum inhibitory concentrations for copper (Cu) and zinc (Zn) were determined by agar dilution method. Those strains that exhibited highest minimum inhibitory concentrations values to the metals (5mM) were subjected to 400-3200 mg/L concentrations of the three mutagenic agents, acriflavine, acridine orange and ethidium bromide. After determination of subinhibitory concentrations, the minimum inhibitory concentrations values for copper and zinc metal ions were again determined, which showed more than 10 fold increase in minimum inhibitory concentrations value (10 mM for Cu and 20 mM for Zn) with P≤0.05. The atomic absorption spectroscopy of dried biomass obtained from resistant strains after exposure to mutagenic agents revealed that strains 13 accumulate the highest amount of intracellular copper (0.35% Cu/mg dried biomass) and strain 10 showed highest accumulation of zinc (0.3% Zn/mg dried biomass) respectively with P≤0.05. From above results it was concluded that the treatment of industrial waste containing heavy metals by artificially mutated bacteria may be appropriate solution for effluent disposal problems.

Keywords
Pollution, bioremediation, copper and zinc waste effluents, pseudomonas

 
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