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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-2630
Vol. 2, No. 2, 2005-2006, pp. 141-145
Bioline Code: st05019
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 2, No. 2, 2005-2006, pp. 141-145

 en Induced mutation and in vitro techniques as a method to induce salt tolerance in Basmati rice ( Oryza sativa check for this species in other resources L.)
M. Y. Saleem, Z. Mukhtar, A. A. Cheema and B. M. Atta

Abstract

Embryogenic callus of indica rice ( Oryza sativa check for this species in other resources L.) cv. Basmati 370 induced on MS medium containing 9.05 μM2, 4-D was irradiated at 50 Gy of gamma rays of 60Co for creating genetic variabilityagainst salinity. Irradiated and non-irradiated calluses were screened in vitro through three consecutive proliferation phases at 4.0, 6.0, 8.0 and 10.0 d/Sm electrical conductivity of NaCl. Growth value and number of adapted mutagenized callus was more than that of non-mutagenized callus. Salinity levels beyond 6 d/Sm and 8 d/Sm were lethal to growth and adaptation of non-irradiated and irradiated callus respectively. NaCl adapted irradiated callus showed 2.0%-4.75% regeneration frequency on MS regeneration medium containing 5.37 M NAA and 9.29 μMKinetin. Non-mutagenizedsalt adapted callus did not showany regeneration. From gamma ray mutagenized cultures, 2 putative lines (M2 generation) with moderate salt tolerance were obtained at seedling stage. The results suggest that in vitro technique in connection with gamma rays may be used as a versatile approach to improve the level of salt tolerance in Basmati rice for saline environment.

Keywords
Rice, induced mutation, NaCl stress, callus, regeneration

 
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