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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-1472
Vol. 2, No. 3, 2005-2006, pp. 201-206
Bioline Code: st05027
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 2, No. 3, 2005-2006, pp. 201-206

 en Nitrate reductase activity: A solution to nitrate problems tested in free and Immobilized algal cells in presence of heavy metals
M. Awasthi

Abstract

Every organism has different potential to accumulate NO3- from the environment. Nitrate reduction processes are perhaps most significant in maintaining water quality by alteration of nitrate to nitrite. A comparative study between the nitrate reductase NR activity of green and blue green algae in presence of heavy metalsis being conducted to present a situation where nitrate reductase process may be affected in presence of heavy metals. Metals interacted negatively with the nitrate reductase activity of a blue green alga, Anacystis nidulans check for this species in other resources and green algae, Chlorella vulgaris check for this species in other resources in both free and immobilized state. The activity was more repressed in C. vulgaris in presence of Ni compared to Zn and Cd. However, Cd was more toxic to NR activity in A. nidulans (free state). Metal dependent variation between free and immobilized cells were found to be significant (P< 0.01) however, the concentration dependent pattern in the activity between free and immobilized state was non significant in both the test organisms. C.vulgaris is more efficient in conversion of nitrate to nitrite compared to A.nidulans in presence of heavy metals.

Keywords
Chlorella. vulgaris, heavy metals, anacystis nidulans, immobilization, nitrate reductase

 
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