Enumeration of Cryptosporidium spp and Giardia spp (oo)cysts in a tropical eutrophic lake: The municipal lake of Yaounde

The biodynamic of Cryptosporidium spp. oocysts and Giadia spp. cysts have been assessed in the municipal lake of Yaounde, which is an artificial eutrophic lake that has been constructed on the Mingoa stream of the Mfoundi River Basin of Cameroon. The oocysts were identified by the Ziehl-Neelsen method while the cysts were identified by the Lugol iodine coloration. The values obtained suggest an increase in (oo)cysts density from the surface to the bottom of the lentic ecosystem. The oocysts varied from 56 oocysts/L in the upper layer to 2640 oocysts/L of water in the lowest layer, while the cysts population dynamics ranged from 24 cysts/L at the surface layer of the lake to 1713 cysts/L at the lowest layers of the lake. There was a considerable reduction in the resistant forms of these emerging pathogenic protozoa between the surface water of the Mingoa stream entering the Lake and that coming out from the Lake. The highest value of Cyptosporidium oocysts determined at the entrance of the lake is 1480 oocysts/L while at the outlet the highest value of oocysts identified is 620 oocysts/L. The highest value of Giardia cysts determined at the entrance of the lake is 352 cysts/L while at the outlet the highest value recorded is 294 cysts/L.

Keywords
Cryptosporidium, oocysts, Giardia, cysts, municipal lake, Yaounde, Cameroon