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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-2630
Vol. 4, No. 4, 2007, pp. 513-520
Bioline Code: st07066
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 4, No. 4, 2007, pp. 513-520

 en Biosynthesis of biodegradable polyhydroxyalkanotes biopolymers in genetically modified yeasts
Abd-El-haleem, D.; Amara, A.; Zaki, S.; Abulhamd, A. & Abulreesh, G.

Abstract

In the recent decade, biosynthesis of the degradable biopolymers polyhydroxyalkanotes in transgenic yeasts became an important research task. Most research strategies depend on either metabolic engineering or molecular approaches. In the present work, research compared PHA biosynthesis in two types of yeasts; Saccharomyces cerevisiae check for this species in other resources and a non-convenient Kloeckera spp.Yeast strains were equipped in their cytoplasm with the phaABCRe operon containing genes phbA, phbB and phbC of the PHA biosynthetic pathway of Ralstonia eutropha check for this species in other resources , which encode â-ketothiolase, NADPH-linked acetoacetyl-CoA reductase and PHA synthase, respectively. The transgenic strains Saccharomyces cerevisiae and Kloeckera sp. were able to produce PHA. The maximum content of the polymer detected in the recombinant strain INVSc1/PHA1 was 2.68 % and only poly-3-hydroxybutyrate (PHB) accumulated. However, the non-conventional transgenic strain KY1/PHA was able to accumulate as maximum of 7.06 % of the copolymer poly-(3-hydroxybutyrateco-poly-3-hydroxyvalerate) (PHV). Western blot analysis confirmed expression of the phaABCReoperonin the transgenic yeast strains. The nature of the PHA thus produced by all tested strains was analyzed by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy.

Keywords
Recombinant yeasts, polyhydroxyalkanotes, poly-3-hydroxybutyrate, nuclear magnetic resonance

 
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