International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
Vol. 7, No. 3, 2010, pp. 457-464
Bioline Code: st10045
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Environment Science and Technology, Vol. 7, No. 3, 2010, pp. 457-464
© Copyright 2010 - Center for Environment and Energy Research and Studies (CEERS)
Decolorization of different azo dyes by Phanerochaete chrysosporium RP78 under optimal condition|
Ghasemi, F.; Tabandeh, F.; Bambai, B. & Sambasiva Rao, K.R.S.
Detoxification of synthetic dyes is one of the main challenges in clearing textile industry wastes.
Biodegradation of azo-dyes using Phanerochaete chrysosporium is one the most environmentally friendly methods
available. The main enzymes responsible for mycodecolorization process are lignin and manganese peroxidases. Here,
optimization of expression conditions has been carried out with manipulating culture condition and nutrient sources.
Therefore, the effects of buffer and temperature as well as nitrogen source on lignin peroxidase and manganese peroxidase
production were investigated at two levels and four levels, respectively. For this purpose, P. chrysosporium RP78
based on Taguchi design of experiment has been applied. Maximum lignin and manganese peroxidase activities of
182 ± 2.5 U/L and 850 ± 41 U/L were obtained under predicted optimum conditions, respectively. Thereby, about
100 % decolorization was achieved after 24 h for two most widely used groups of azo dyes in textile industry consisting
reactive and acidic. The physical adsorption of the azo dyes by mycelia was not significant which indicated that the
enzymatic degradation of the dyes was occurred. Time profile of these enzymes showed that manganese peroxidase was
peaked on 9th day while lignin peroxidase peaked on 13th. day and remained stable in the culture. The extracellular
expression profiles of both were studied by 2 dimensional gel electrophoresis to partially characterize the enzymes.
Biodegradation; Lignolytic enzymes; Phanerochaete chrysosporium; Taguchi method
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