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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-2630
Vol. 8, No. 1, 2011, pp. 45-56
Bioline Code: st11004
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 8, No. 1, 2011, pp. 45-56

 en Biodegradation of cypermethrin by a newly isolated actinomycetes HU-S-01 from wastewater sludge
Lin, Q.S.; Chen, S.H.; Hu, M.Y.; Ul Haq, M.R.; Yang, L. & Li, H.

Abstract

A new cypermethrin degrading strain was isolated from wastewater sludge using enrichment technique. On the basis of morphological, physiological, biochemical characteristics tests and 16S rDNA sequence analysis, the strain was determined to be a Streptomyces check for this species in other resources species, probably a strain of Streptomyces parvulus check for this species in other resources , so it was designated as Streptomyces sp. HU-S-01. The strain Streptomyces sp.HU-S-01 is aerobic and optimum growth temperature for the strain was found to be 26-28°C with initial pH range 6.0-9.0 and pH 7.5 was found to be the optimum. This strain can also completely degrade 3-phenoxybenzoic acid within 96 h at the concentration of 50 mg/L. The kinetic constants Vmax, Km, Kcat and Kcat/ Km of enzyme for cypermethrin were 1.236 μmol/min, 6.418 μmol/mL, 13.493 min and 2.102 mL/mol.min, respectively. The degradation products of cypermethrin were identified using gas chromatograph-mass spectrometric (GC-MS) analysis. The degradation pathway followed by HU-S-01 involves oxidative as well as hydrolyzing. Biodegradation ability of strain Streptomyces sp. HU-S-01 without toxic byproducts reveals its potential for further study as a biological agent for the remediation of soil, water or crops, contaminated with cypermethrin.

Keywords
Biodegradation; Metabolites; Pyrethroid insecticides; Streptomyces sp.; 3-phenoxybenzoic acid

 
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