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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-2630
Vol. 8, No. 4, 2011, pp. 831-840
Bioline Code: st11077
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 8, No. 4, 2011, pp. 831-840

 en Characterization of bioflocculants produced by bacteria isolated from crude petroleum oil
Zaki, S.; Farag, S.; Abu Elreesh, G.; Elkady, M.; Nosier, M. & Abd El Haleem, D.

Abstract

The aim of this study was to look for high efficient bioflocculant-producing microorganisms. Among 36 bacterial colonies isolated from a crude petroleum oil sample, three of them as Bacillus subtilis check for this species in other resources and Pseudomonas check for this species in other resources spp. exhibited flocculation activity exceeding 90% after 3 days of cultivation. They were identified by 16 S rDNA sequence analysis as Bacillus subtilis and Pseudomonas sp. Spectroscopic analysis of the polymers by nuclear magnetic resonance and fourier-transform infrared revealed that the polymers were glycoproteins. These polymers were soluble in water and insoluble in any organic solvents tested. The effects of bioflocculant dosage, temperature and pH on the flocculation activity were evaluated. The maximum bioflocculation activities were observed at an optimum bioflocculant dosage of 3.5 mg/L (strains Bacillus subtilis and Pseudomonas) and 5.0 mg/L (strain CPO14), respectively. In addition, these biopolymers were able to flocculate kaolin suspension (5 g/L) over a wide range of pH (pH 3-9) and temperature (5–50°C) tested in the presence of CaCl2. The highest flocculation activities of strains CPO8, CPO13 and CPO14 were 96.03%, 92.17% and 97.59%, respectively in the early stationary phase (at 24 h), while the cell production reached its maximum in the stationary phase (at 72 h). Their efficient flocculation capabilities suggest potential applications in industries.

Keywords
Bioflocculant; Biopolymer; Flocculation activity; Optimization

 
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