search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-2630
Vol. 10, No. 1, 2013, pp. 181-190
Bioline Code: st13020
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 10, No. 1, 2013, pp. 181-190

 en Response surface methodology mediated optimization of Remazol Orange decolorization in plain distilled water by Pseudomonas aeruginosa check for this species in other resources BCH
Jadhav, S.B.; Surwase, S.N.; Phugare, S.S. & Jadhav, J.P.

Abstract

The bacterium Pseudomonas aeruginosa check for this species in other resources BCH decolorized and degraded the sulphonated azo dye Remazol Orange in plain distilled water. The effects of different parameters, i.e. pH, temperature and cell mass concentration on the biodegradation of dye in aqueous phase was evaluated using response surface methodology. Optimization was carried out using three-level Box–Behnken design. Predicted values were found to be in good agreement with experimental values (R2 0.9997 and pred R2 0.9984), which indicated suitability of the employed model and the success of response surface methodology. Optimum dye decolorization was maximized and the favourable conditions were pH 7.43, temperature 29.39 °C and cell mass concentration 2.88 g l-1, which resulted in 96.01 % decolorization within 5 h. It was validated from the predicted response (97.37 %). According to the analysis of variance results, the proposed model can be used to navigate the design space. 3D plot analysis disclosed the significant interaction between all three tested factors on decolorization process. The combinations of the three variables predicted during response surface methodology were confirmed through confirmatory experiments. Observations indicated that higher cell mass accelerated the decolorization process. Degradation of the dye was verified through high performance liquid chromatography analysis. Phytotoxicity studies carried out with dye and dye metabolites using Phaseolus mungo check for this species in other resources , Triticum aestivum check for this species in other resources and Sorghum vulgare check for this species in other resources indicated the detoxification of dye.

Keywords
Biodegradation; Box-Behnken design; High performance liquid chromatography; Phytotoxicity

 
© 2013 - Center for Environment and Energy Research and Studies (CEERS)
Alternative site location: http://www.ijest.org

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil