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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-2630
Vol. 11, No. 2, 2014, pp. 367-376
Bioline Code: st14038
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 11, No. 2, 2014, pp. 367-376

 en Application of indigenous microbial consortia in bioremediation of oil-contaminated soils
Shankar, S.; Kansrajh, C.; Dinesh, M. G.; Satyan, R. S.; Kiruthika, S. & Tharanipriya, A.

Abstract

Bioremediation of oil spillage in soils using consortia of microbes beckons much exploration. The present study involves bioremediation of oil-contaminated soils from north Chennai, India, using indigenous microbial consortia. Totally, 32 positive oil degrading isolates were obtained from 3 different locations, i.e., petrol filling stations, automobile workshops and oil refineries. Substrate utilization patterns of individual isolates and the consortial sets were observed. Mixture of three common hydrocarbons (petrol, diesel and engine oil) was used for studies. The substrate oil utilized by consortia was taken for thin-layer and column chromatography which perfectly resulted in varied fractions of oil compared to the unused oil as control. The best consortia were used directly for bioremediation experiment. Three different oil-contaminated soils were used and bioremediation patterns were observed. The rate of bioremediation differed within soils, nevertheless all soils were almost 100 % reclaimed within 30 days. Bioremediation kinetics showed that the process corresponds to first-order kinetics and kinetic constants for the different soils ranged from 0.051 to 0.077/day. Assessment of detoxification of acute phytotoxicity owing to the pollutant oil was done, and results observed were significant. An increase of 25, 300 and 212 % in germination index, average growth index and sustenance index, respectively, of Trigonella foenumgraecum check for this species in other resources Linn. in treated soils was observed, compared to untreated soils. Thus, this study confirmed that microbes in ‘Consortial Union’ serve as better treating agents in bioremediation of oil-contaminated soils than individual microorganisms.

Keywords
Trigonella foenum-graecum; Hydrocarbons; Kinetics; Phytotoxicity

 
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