Production of microbial oil has attracted a great
attention in recent years. The potential of lipid production
by the yeast strains is the reason for using microorganisms
for biodiesel production. Microbial lipid has high similarity
to the oil obtained from plants and animals in type and
composition. Production of oil from yeasts must be economical,
so optimization of the cultivation condition to
reach higher production must be done. Native oleaginous
yeast,
Cryptococcus albidus
, was isolated from soil by the
nitrogen-limited medium and screened by Nile red staining.
Yarrowia lipolytica
DSM 8218 was used for lipid production
as a standard strain.
C. albidus was an excellent
oleaginous yeast, and the lipid quantity, dry biomass and
lipid productivity of this strain were 11.81 g/l, 19.65 g/l
and 60.1 %, respectively, in shaking flask cultivation at
150 rpm and 25
oC in nitrogen-limited medium containing
per liter 75 g glucose, 1 g (NH
4)2SO
4, 1 g yeast extract,
3 g KH
2PO
4, 1.5 g MgSO
4.7H
2O, 0.15 g CaCl
2, 0.06 g
MnSO
4.H
2O, 0.02 g ZnSO
4.7H
2O and 0.15 g FeCl
3.6H
2O
with pH adjusted to 6.5. Fourier transform infrared spectroscopy
was used for analyzing and confirming the production
of microbial oil in this study.
