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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-2630
Vol. 11, No. 3, 2014, pp. 685-694
Bioline Code: st14067
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 11, No. 3, 2014, pp. 685-694

 en Specific detection of bioavailable phenanthrene and mercury by bacterium reporters in the red soil
Wei, H.; Ze-Ling, S.; Le-Le, C.; Wen-hui, Z. & Chuan-Chao, D.

Abstract

Genetically engineered Pseudomonas putida check for this species in other resources reporters (BMB-PL and BMB-ME), which, respectively, carried phnS-luxCDABE and merR-egfp cassette,were used to determine bioavailable phenanthrene and mercury. Over a spiked range of concentrations and aged for 6 days in red soil samples, the reporters were tested to determine the optimal assay conditions and the bioavailable phenanthrene (0–60 mg kg-1) and Hg2+ (0–240 μg kg-1) were evaluated by the signal of the relative fluorescent units and relative luminescence units. Single contaminationwas carried out and good correlations were obtained between signal strength and pollutant concentrations, whereas interference and bioavailability repression were observed in dual-contamination experiments. Other heavy metal ions at nanomolar level did not interfere with BMB-ME measurement while BMB-PL showed some response to other polycyclic aromatic hydrocarbons or their intermediate products during degradation. Comparing high-performance liquid chromatography methods with the bacterial reporters, both BMB-MEand BMB-PL appeared to have a detection limit (mercury <40 μg kg-1; phenanthrene <24 mg kg-1) similar to the instrumental analysis. Although physical parameters may affect the interaction of pollutants with bioreporter cells, advantages include the inherent biological relevance of the response, rapid response time, and potential for field deployment. Our results strongly suggest that theBMB-ME andBMB-PL bioreporters constitute an adaptable system for easily detecting the bioavailability of mercury and phenanthrene in the red soils.

Keywords
Biosensor; Contamination; Pseudomonas putida; Red soil

 
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