search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-2630
Vol. 11, No. 4, 2014, pp. 1127-1134
Bioline Code: st14111
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 11, No. 4, 2014, pp. 1127-1134

 en Optimization of novel hyperthermostable β amylase production by Bacillus subtilis check for this species in other resources DJ5 using solid agroresidual substrates
Poddar, A. & Jana, S. C.

Abstract

Eight different low cost starchy agroresidues namely Barley (B), Wheat bran (WB), Sattu (S), Rice powder (RP), Corn flour (CF), Rice husk (RH), Yellow peas split (YPS) and arrowroot (A) were used for solid culturing of Bacillus subtilis check for this species in other resources DJ5 for production of novel hyperthermostable β amylase. Various process parameters like initial moisture content, inoculum load, medium pH and incubation temperature affecting enzyme production were optimized to ensure maximum enzyme yield. Only 10 % inoculums load and medium pH of 6.9 was found sufficient to achieve maximum enzyme production in all substrates in a decreasing order, B>WB>S>RP> CF>RH>YPS>A. Optimum β amylase production was highly dependent on initial moisture content of substrate as observed from varying requirement of moisture for different substrates. Only 50 % moisture was sufficient for maximum enzyme production of 84.29 U/gdm in CF. For B, RH, YPS, and A 60 % initial moisture resulted in higher production of 120.34, 35.19, 26.59, and 21.58 U/ gdm, respectively, at 37 °C. However, for S and RP higher (70 %) moisture content allowed 113.4 and 85.56 U/gdm enzyme production, respectively. Under optimized conditions, maximum β amylase production was observed after 25 h for A, YPS, RH, RP; 41 h for B, WB, CF, and 45 h for S.

Keywords
Agroresidues; Barley; Moisture content; Wheat bran

 
© International Journal of Environment Science and Technology
Alternative site location: http://www.ijest.org

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 05-Dec-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil