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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-2630
Vol. 11, No. 6, 2014, pp. 1571-1580
Bioline Code: st14153
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 11, No. 6, 2014, pp. 1571-1580

 en Biodegradation of benzo(a)pyrene mediated by catabolic enzymes of bacteria
Mishra, S. & Singh, S.N.

Abstract

An investigation was carried out to compare the ability of two bacteria Pseudomonas aeruginosa check for this species in other resources PSA5 and Rhodococcus check for this species in other resources sp. NJ2 isolated from petroleum sludge for degradation of benzo(a)pyrene [B(a)P], a HMW PAH compound in MSM. During 25 days of incubation, 50 ppm B(a)P was degraded by 88 and 47 % by P. aeruginosa PSA5 and Rhodococcus sp. NJ2, respectively. Besides, involvement of different catabolic enzymes, that is, salicylate hydroxylase, 2-carboxybenzaldehyde dehydrogenase, catechol 1,2-dioxygenase and catechol 2,3-dioxygenase, was also examined to identify their differential role in B(a)P degradation. Among these enzymes, the highest induction of 2-carboxybenzaldehyde dehydrogenase (773.5 nmol mg-1 protein) was recorded in P. aeruginosa PSA5, while salicylate hydroxylase was highly expressed (839.6 nmol mg-1 protein) in Rhodococcus sp. NJ2. Both the bacteria were found biosurfactant (glycolipid) producing, and role of biosurfactant in PAH degradation was also ascertained by reduced surface tension, higher emulsification index and increased cell surface hydrophobicity.

Keywords
Biosurfactant; Cell surface hydrophobicity; Degradative enzymes; Surface tension

 
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