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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-2630
Vol. 11, No. 7, 2014, pp. 1845-1854
Bioline Code: st14180
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 11, No. 7, 2014, pp. 1845-1854

 en Microbial community analysis for aerobic granular sludge reactor treating high-level 4-chloroaniline wastewater
Zhu, L.; Dai, X.; Xu, X.; Lv, M. & Cao, D.

Abstract

A laboratory-scale sequencing airlift bioreactor continuously treating high-level 4-chloroaniline (4-ClA) wastewater was used for studying the effect of 4-ClA on the characteristics and microbial community of aerobic granular sludge. The granulation of aerobic sludge and efficient pollutant removal performance were developed via shortening sludge settling time and gradually increasing influent 4-ClA concentration to around 400 mg L-1. However, the granular sludge reactor deteriorated with the 4-ClA loading rate above 0.8 kg m-3 d-1. Denaturing gradient gel electrophoresis and real-time quantitative PCR were applied to investigate the microbial community succession during the start-up and recovery of bioreactor. The results showed that the performance of granular reactor was significantly influenced by the microbial community of aerobic granule, and stable aerobic granule was dominated with β-Proteobacteria (61.28 %), Flavobacteriales, Planctomycetales, Clostridiales, and Acidobacteria. Since Thauera check for this species in other resources (21.55 %) related to the genus β-Proteobacteria was abundant in the stable 4-ClA-degrading granular sludge, it was speculated as the main 4-ClA-degrading bacteria. Under high chloroaniline level, the sludge granulation may maintain the stability of the bioreactor via adjusting the composition of microbial community and abundance of functional microorganism. This paper provided useful information for better understanding the change of microbial community characteristics under highlevel toxic organic pollutants and process optimizing.

Keywords
Aerobic granular sludge; 4-Chloroaniline (4- ClA); Microbial community; Succession; Thauera sp.

 
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