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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-2630
Vol. 11, No. 7, 2014, pp. 1939-1946
Bioline Code: st14190
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 11, No. 7, 2014, pp. 1939-1946

 en Radiation resistant of native Deinococcus check for this species in other resources spp. isolated from the Lout desert of Iran ‘the hottest place on Earth’
Mohseni, M.; Abbaszadeh, J. & Nasrollahi Omran, A.

Abstract

Two native ionizing radiation-resistant bacteria were isolated and identified from a soil sample collected from extreme conditions of the Lout desert in Iran. The hottest land surface temperature has been recorded in the Lout desert from 2004 to 2009. Also, it is categorized as a hyper arid place. Both ionizing radiation and desiccation may cause damage on genome. Soil sample was irradiated in order to eliminate sensitive bacteria then cultured in onetenth- strength tryptic soy broth medium. Bacterial suspension used for radiation treatment. Morphological and physiological characterization and phylogenetic studies based on 16S rRNA gene sequence were used for identification. The cells were rod shape, non-motile, non-spore forming and gram positive. The 16S rRNA gene sequence showed 99.5 % of similarity to Deinococcus ficus check for this species in other resources . Phylogenetic dendrogram demonstrated that the isolates branched with Deinococcus xibeiensis check for this species in other resources , D. ficus and Deinococcus mumbaiensis check for this species in other resources . Both isolates were resistant to >15 kGy of gamma radiation and >600 J m2 of UV radiation. This is the first report on radiation resistant bacteria belonging to genus Deinococcus isolated from the Lout desert of Iran.

Keywords
Deinococcus; Radiation-resistant bacteria; Ionizing radiation; Lout desert

 
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