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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472 EISSN: 1735-1472
Vol. 12, No. 6, 2015, pp. 1889-1898
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Bioline Code: st15176
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Environment Science and Technology, Vol. 12, No. 6, 2015, pp. 1889-1898
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Hydrocarbon pollution does not influence bacterial diversity as much as geographic location: a Korean case study
Cho, D.-H.; Baek, K.-H.; Ramanan, R.; Ahn, C.-Y.; Ahn, K.-H.; Yoon, B.-D.; Oh, H.-M. & Kim, H.-S.
Abstract
Bacterial diversity of hydrocarbon-contaminated
sites in various regions of Korea was investigated
to ascertain the influence of hydrocarbon pollution on
bacterial diversity using terminal restriction fragment
length polymorphism (T-RFLP) and differential gel gradient
electrophoresis. Thirty-two hydrocarbon-contaminated
soil samples were collected from seven different
geographical locations in Korea. A dendrogram of
T-RFLP profiles for the bacterial community structure in
soil samples using Ward’s method with Jaccard distance
showed that samples from the same location clustered
together. Principal components analysis (PCA) and selforganizing
maps (SOM) of terminal restriction fragments
were also used to characterize the associations among
samples. PCA and SOM results also showed that soil
bacterial communities were classified according to locations,
but not by hydrocarbon pollution level. Moreover,
correlation analyses prove a direct correlation between
bacterial diversity and meteorological parameters,
whereas no significant correlation was observed with
hydrocarbon contamination levels. These results suggest
that geographical origin, rather than soil contamination
level, might be more important in determining the bacterial
diversity of crude oil-contaminated soils. Environmental
factors, which play a major role in determining
natural bacterial diversity which in turn should be enriched
for effective bioremediation, should be the central
dogma while considering bioremediation of hydrocarbon-contaminated
sites.
Keywords
Soil; Bacterial diversity; Crude oil; Contamination; Geographic location; Statistical analysis
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