search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-2630
Vol. 12, No. 7, 2015, pp. 2161-2168
Bioline Code: st15203
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 12, No. 7, 2015, pp. 2161-2168

 en Biodegradation of azo dye Direct Orange 16 by Micrococcus luteus check for this species in other resources strain SSN2
Singh, S.; Chatterji, S.; Nandini, P. T.; Prasad, A. S. A. & Rao, K. V. B.

Abstract

In the present study, the decolorization and degradation of azo dye Direct Orange 16 (DO-16) by a potential bacterial isolate isolated from textile effluent were evaluated. Through 16S rRNA sequence matching, the potential isolate was identified as Micrococcus luteus check for this species in other resources strain SSN2. The effects of various factors (pH, temperature, salt and dye concentration) on decolorization were investigated. The strain SSN2 had the ability to decolorize DO-16 with 96 %efficiency at pH 8, 37 °C and 3 % NaCl in a short time of 6 h under static conditions. DO-16 decolorization was assessed by UV–Vis spectrophotometer with gradual decrease of dye peak intensity at 430 nm (λmax). Analytical techniques (thin-layer chromatography, Fourier transform infrared spectroscopy and high-performance liquid chromatography) further confirmed that biodegradation of DO- 16 was due to reduction of the azo bond. The phytotoxicity assay (with respect to seeds of Vigna mungo check for this species in other resources and Vigna radiata check for this species in other resources ) demonstrated the less toxic nature of the DO-16- degraded products compared to the toxic azo dye.

Keywords
Dye; Bacterial strain; Decolorization; Degradation; Phytotoxicity

 
© Copyright 2015 - International Journal of Environment Science and Technology
Alternative site location: http://www.ijest.org

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 05-Dec-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil