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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472 EISSN: 1735-1472
Vol. 12, No. 7, 2015, pp. 2253-2262
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Bioline Code: st15212
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Environment Science and Technology, Vol. 12, No. 7, 2015, pp. 2253-2262
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Self-immobilised bacterial consortium culture as ready-to-use seed for crude oil bioremediation under various saline conditions and seawater
Kok Kee, W.; Hazaimeh, H.; Mutalib, S. A.; Abdullah, P. S. & Surif, S.
Abstract
Biodegradation of crude oil hydrocarbon by
microorganisms in seawater is generally slow because of
the harsh environmental condition due to high salinity. The
aim of this study was to compare sawdust (SD) and oil
palm empty fruit bunch wastes as suitable carrier material
to immobilise hydrocarbon-degrading bacterial consortium
culture to accelerate and improve crude oil degradation in
seawater. The consortium culture was found able to tolerate
salinity up to 3 %, where the degradation of crude oil was
not inhibited (p> 0.05). In artificial seawater, suspension
of bacterial consortium culture was able to degrade
83.3 ± 3.00 % of crude oil within 8 weeks, which indicated
the possibility of using consortium culture in seawater.
When tested in seawater, suspension of consortium
culture managed to degrade 47.7 ± 1.53 % of crude oil in
8 weeks. In order to improve the performance of consortium
culture, immobilisation of consortium culture onto SD
and oil palm empty fruit bunch was successfully undertaken
when formation of biofilm layers was observed under
scanning electron microscope. Immobilising consortium
culture onto oil palm empty fruit bunch and SD was shown
to increase crude oil biodegradation to 68.7 ± 4.04 and
62.3 ± 5.51 % in 8 weeks, respectively. This study demonstrated
immobilisation of consortium culture onto SD
and oil palm empty fruit bunch can be utilised as ready-touse
seeds to improve and accelerate crude oil biodegradation
in seawater.
Keywords
Bacteria; Biodegradation; Biofilm; Carrier materials; Hydrocarbon; Petroleum wastes
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