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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-2630
Vol. 12, No. 8, 2015, pp. 2757-2764
Bioline Code: st15258
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 12, No. 8, 2015, pp. 2757-2764

 en Optimizing the nutrient feeding strategy for PHA production by a novel strain of Enterobacter check for this species in other resources sp.
Vinish, V.; Sangeetha, S. H.; Aravind, J.; Kanmani, P. & Sathiskumar, T.

Abstract

The influence of nutrient limitation on polyhydroxyalkanoate (PHA) accumulation was studied using a novel PHA-producing strain of Enterobacter check for this species in other resources sp. The effect of N/C ratio on growth and accumulation of PHA was studied by varying the ratio from 0.02 to 0.1. Biomass concentration, dry cell weight, protein content and the amount of PHA accumulated were estimated for each N/C ratio. It was found that the increase in N/C ratio resulted in increase in culture concentration up to 3.25 g/l of dry cell weight. Polyhydroxyalkanoate concentration was found to be maximum at N/C ratio of 0.04 (67.8 μg/ml), and further increase in N/C ratio resulted in lesser amount of PHA. Analytical procedures such as FTIR and NMR were done to validate the obtained PHA biopolymer.

Keywords
PHA; Novel strain; Enterobacter sp.; Nutrient limitation; N/C ratio; FTIR; NMR

 
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