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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-2630
Vol. 12, No. 10, 2015, pp. 3263-3272
Bioline Code: st15307
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 12, No. 10, 2015, pp. 3263-3272

 en Study on cellular changes and potential endotrophy of wheat roots due to colonization of Chromium reducing bacteria
Batool, R.; Yrjälä, K. & Hasnain, S.

Abstract

Chromium is a highly toxic metal for all living organisms. Industrial use of chromium has resulted in serious widespread pollution. Biological treatment (bioremediation) has proven to be a cost-effective option for cleanup of metal-contaminated sites. Several bacteria and plant species are able to tolerate high levels of chromium compounds that can be used for cleanup. An experiment was designed to study the colonization behavior of two indigenous Cr(VI)-reducing bacterial strain Pseudomonas aeruginosa check for this species in other resources Rb-1 and Ochrobactrum intermedium check for this species in other resources Rb-2 that were grown in wheat system amended with and without Cr(VI). Hydroponically grown wheat seedlings were coinoculated with bacterial cultures to study the root colonization potential by fluorescent and electron microscopy. Bacterial inoculation caused significant increase in the growth of seedlings under Cr(VI) stress. Fluorescent microscopy showed good colonization potential of both bacterial strains with roots of inoculated seedlings. Electron micrographs revealed that Rb-1 tended to accumulate in the form of clusters, while Rb-2 preferred to be attach in groups of two or three cells to the root surface of inoculated seedlings. Chromium stress led to the elongation of bacterial rods along with uneven cell surface due to wrapping of cells in mucilaginous material. Cr(VI) stress also resulted in the damaging of plant root surface. Hence, few cells of Rb-2 entered the damaged root cortex cells and appeared as endophytes. Excessive production of fibrillar material by both bacteria under chromium stress could clearly be observed. Both strains displayed auxin production and Cr(VI) reduction ability, showing promise for bioremediation purposes.

Keywords
Cr(VI) reducing bacteria; Artificial association; Electron microscopy; Triticum aestivum

 
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