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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-2630
Vol. 12, No. 12, 2015, pp. 3781-3790
Bioline Code: st15354
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 12, No. 12, 2015, pp. 3781-3790

 en Fungi colonizing wood sticks of Chinese fir incubated in subtropical urban soil growing with Ficus microcarpa check for this species in other resources trees
Ding, S.; Hu, H. & Gu, J.-D.

Abstract

Fungi colonized on wood sticks of the Chinese fir Cunninghamia lanceolata check for this species in other resources after incubation in soil around the Ficus microcarpa check for this species in other resources trees were isolated. In total, 1189 wood sticks from 28 F. microcarpa trees were sampled and analyzed in this study. A combined approach of culture morphological observation and PCR amplification of ITS of purified fungal isolates in revealing restriction fragment length polymorphism patterns was applied to group the purified fungal isolates for further species identification. Fungal species were identified based on phylogenetic analysis of ITS and β-tubulin gene-amplified sequences. Overall, 25 species belonging to 17 genera from four classes were identified, and all of them were Ascomycota. Among them, Penicillium check for this species in other resources spp., Fusarium check for this species in other resources spp., and Phoma insulana check for this species in other resources were the most frequently isolated. In this study, Dichotomonyces cejpii, Fusicolla acetilerea check for this species in other resources , and Purpureocillium lilacinum check for this species in other resources are recorded in Hong Kong for the first time. Some species of the fungal cultures obtained in this study may have potential application as biocontrol agents to control tree disease.

Keywords
ITS; RFLP; Soil fungi; Taxonomy; Urban environment

 
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