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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-2630
Vol. 12, No. 12, 2015, pp. 3887-3894
Bioline Code: st15366
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 12, No. 12, 2015, pp. 3887-3894

 en Effects of two plant growth-promoting rhizobacteria containing 1-aminocyclopropane-1-carboxylate deaminase on oat growth in petroleum-contaminated soil
Liu, J. L.; Xie, B. M.; Shi, X. H.; Ma, J. M. & Guo, C. H.

Abstract

Petroleum is potentially toxic to living organisms, and there are worldwide efforts to develop methods for bioremediation of petroleum-contaminated soils. Phytoremediation is an effective method to reduce the concentration of petroleum in soils, and plant growthpromoting rhizobacteria (PGPR) play an important role in the phytoremediation. Two PGPR, Pseudomonas aeruginosa check for this species in other resources SLC-2 and Serratia marcescens check for this species in other resources BC-3, were isolated from the rhizophere of Echinochloa check for this species in other resources grown in petroleumcontaminated soil. These isolates showed capacities for 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, indole acetic acid production, siderophore synthesis, and the degradation of petroleum. The ACC deaminase activity of SLC-2 and BC-3 was 2.52 ± 0.03 µmol α-KA (mg Pr∙h)-1 and 38.52 ± 0.37 µmol α-KA (mg Pr∙h)-1, respectively. On the other hand, when the concentration of L-Trp increased, the IAA synthesis of BC-3 also increased, while the synthesis of SLC-2 did not change significantly. The ability of synthesized siderophore of SLC-2 was much higher than that of BC-3. The petroleum degradations of SLC-2 and BC-3 increased 4.78 and 7.36 %, respectively. The pot experiment of oat was performed to evaluate the plant growth-promoting abilities of SLC-2 and BC-3. Compared with non-inoculated controls, the height and fresh weight of stems increased (23.64 and 42.57) % and (16.98 and 28.3) %, respectively, whereas the length and fresh weight of roots also increased (10.34 and 20.84) and (24.13 and 43.11) %, respectively, when inoculated with SLC-2 and BC-3. The results indicated that P. aeruginosa SLC-2 and S. marcescens BC-3 can serve as promising microbes for increasing plant growth in petroleum-contaminated soil to improve the phytoremediation efficiency.

Keywords
Plant growth-promoting rhizobacteria (PGPR); 1-Aminocyclopropane-1-carboxylate (ACC) deaminase; Petroleum; Phytoremediation

 
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