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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 2, No. 3, 2005, pp. 238-243
Bioline Code: tc05025
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 2, No. 3, 2005, pp. 238-243

 en Research communication- ANTIBACTERIAL SCREENING OF FOUR LOCAL PLANTS USING AN INDICATOR-BASED MICRODILUTION TECHNIQUE
Umeh, E.U., Oluma, H.O.A., and Igoli, J.O.

Abstract

The antibacterial activities of the plants, Mitragyna inermis check for this species in other resources , Morinda lucida check for this species in other resources , Khaya senegalensis check for this species in other resources and Nauclea latifolia check for this species in other resources were investigated using an indicator-based microdilution technique. The extracts of the plants in water, methanol, chloroform and petroleum ether inhibited growth of bacteria in broth cultures. M. inermis with the lowest minimal inhibitory concentration (MIC) of 0.03g/ml was the bacteriologically most active plant. The chi-square value (10.25) and F-statistic value (4.36) were significant at less than 0.05 level and implied that inhibition of bacterial growth was significantly associated with the type of plant investigated. The R value obtained on regressing bacterial inhibition on the independent variables - type of plant, plant part (leaf or stem bark) and extraction solvent used - was 0.57 (p < 0.01 level), and means that the independent variables put together influenced inhibition of bacterial growth . The findings of this study suggest the effectiveness of the microdilution technique in the phytochemical screening of plants for antibacterial activities.

Keywords
indicator-based, microdilution technique, antibacterial, Mitragyna inermis, Morinda lucida, Khaya senegalensis, Nauclea latifolia.

 
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