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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 4, No. 2, 2007, pp. 231-239
Bioline Code: tc07020
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 4, No. 2, 2007, pp. 231-239

 en SCREENING OF TWENTY-FOUR SOUTH AFRICAN COMBRETUM check for this species in other resources AND SIX TERMINALIA check for this species in other resources SPECIES (COMBRETACEAE) FOR ANTIOXIDANT ACTIVITIES.
P. Masokoa and J.N. Eloff

Abstract

The dried leaves of Combretum check for this species in other resources and Terminalia check for this species in other resources species (Combretaceae)were extracted with acetone, hexane, dichloromethane and methanol. Thin layer chromatography (TLC) plates were developed under saturated conditions and sprayed with 0.2% 2,2-diphenyl-1-picryl hydrazyl (DPPH) in methanol for antioxidant screening. Visualization of separated bands exhibiting antioxidant activities enabled the localization and the subsequent identification of the potential active compounds. The acetone and methanol extracts displayed the presence of antioxidant activity after spraying the chromatogram with DPPH. Hexane and dichloromethane extracts did not have any antioxidant activity. C. hereroense had the highest number of active compounds, followed by C. collinum ssp. taborense, which were 16 and 10, respectively. Acetone extracts of all tested Combretum species had 53 active bands and methanol had 55. All Terminalia species extracted with acetone and methanol had antioxidant activity. T. gazensis and T. mollis methanol extracts had 11 and 14 active compounds respectively in one of the solvent systems used. The qualitative DPPH assay on TLC was successfully used in this study to systematically assess the total antioxidant activity of the Combretum and Terminalia species extracts.

Keywords
Combretaceae; Terminalia species; Combretum species; Antioxidant; 2,2, diphenyl-1-picrylhydrazyl (DPPH)

 
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