Direct effects of Nauclea latifolia
extracts on different gastrointestinal nematodes of sheep is described. In vivo and in vitro
studies were conducted to determine possible anthelmintic effect of leaf extracts of Nauclea latifolia
toward different ovine gastro intestinal nematodes. A larval development assay was used to investigate in vitro
, the effect of aqueous and ethanolic extracts of N. latifolia
towards strongyles larvae. The development and survival of infective larvae (L3
) was assessed and best-fit LC50
values were computed by global model of non-linear regression analysis curve-fitting (95% CI). Twenty sheep harbouring naturally acquired gastrointestinal nematodes were treated with oral administration of ethanolic extracts at a dose rate of 125 mg/kg, 250 mg/kg and 500mg/kg to evaluate therapeutic efficacy, in vivo
The presence of the extracts in the cultures decreased the survival of larvae. The LC50
of aqueous and ethanolic extract were 0.704 and 0.650 mg/ml respectively and differ significantly (P<0.05, paired t test). Faecal egg counts (FEC) on day 12 after treatment showed that the extract is effective, relative to control (1-way ANOVA, Dunnett's multiple comparison test), at 500mg/kg against Haemonchus
spp (p<0.05), Strongyloides
spp (P < 0.01); at 250mg/kg against Trichuris
spp (P < 0.01) and ineffective against Oesophagostomum
spp (p>0.05). The effect of doses is extremely significant; the day after treatment is sometimes significant while interaction between dose and day after treatment is insignificant (2-way ANOVA).
extract could therefore find application in the control of helminth in livestock, by the ethnoveterinary medicine approach.