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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 5, No. 2, 2008, pp. 187-193
Bioline Code: tc08026
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 5, No. 2, 2008, pp. 187-193

 en Antimicrobial Activity off Essential Oils of Ocimum Gratissimum check for this species in other resources L. From Different Populations of Kenya
Matasyoha, Lexa G.; Matasyoh, Josphat C.; Wachira, Francis N.; Kinyua, Miriam G.; Thairu Muigai, Anne W. & Mukiama, Titus K.

Abstract

Hydro-distilled volatile oils from the leaves of Ocimum Gratissimum check for this species in other resources L. (Lamiaceae) of 13 populations of different silvicultural zones were evaluated for antimicrobial activity against Gram positive ( Staphylococcus aereus check for this species in other resources , Bacillus check for this species in other resources spp.) and Gram negative ( Escherichia coli check for this species in other resources , Pseudomonas aeruginosa check for this species in other resources , Salmonella typhi check for this species in other resources , Klebisiella pneumoniae check for this species in other resources , Proteus mirabilis check for this species in other resources ) bacteria and a pathogenic fungus, Candida albicans check for this species in other resources . All the essential oils are active to the tested microbiles with different strength. The highest antimicrobial activity against Gram positive bacteria (Staphylococcus aureus) and Gram negative bacteria (Pseudomonas aeruginosae and Proteus mirabilis) was observed from the eastern Kenya (Meru) oil. Meru oil was the best and its effectiveness was consistent on nearly all the microbes tested. The oil from the plant growing in the coastal region of Kenya (Mombasa) showed the best effect only on Gram negative bacteria (Escherichia coli and Proteus mirabilis). Both oils (Meru and Mombasa) were dominated by monoterpenes accounting for 92.48 % and 81.37 % respectively. The monoterpene fraction was characterized by a high percentage of eugenol (68.8 %) for Meru oil and 74.10 % for Mombasa oil. The other major monoterpene was methyl eugenol (13.21 %). Camphor (0.95 %) was observed only in the Meru oil. (Cis)-Ocimene, (trans)-ocimene and β-pinene were present in both Meru and Mombasa oils. The sesquiterpenes present in fairly good amounts in both oils were germacrene D and (trans)-caryophyllene. The minor sesquiterpenes were α-farnesene (0.85 %) and β-bisabolene (0.74 %) which were present in the Meru oil only.

Keywords
Antimicrobial activity, Ocimum gratissimum L., Eugenol, Essential oil

 
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