search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 6, No. 1, 2009, pp. 42-48
Bioline Code: tc09006
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 6, No. 1, 2009, pp. 42-48

 en Antimicrobial Activity Of Pentacyclic Triterpenes Isolated From Acacia mellifera check for this species in other resources
Mutai, C.; Bii, C.; Rukunga, G.; Ondicho, J.; Mwitari, P.; Abatis, D.; Vagias, C.; Roussis, V. & Kirui, J.

Abstract

Acacia mellifera check for this species in other resources has been used widely in traditional African medicines against various diseases. Among the Kipsigis community of Kenya, water extracts from the plant is used for the treatment of skin diseases, coughs and gastrointestinal ailments. The aim of the study was to provide scientific rationale for the use of the plant in traditional medicine through bioassay-guided fractionation of A. mellifera stem bark. Bioactivity testing was done against selected microbes using disc diffusion technique as outlined in Clinical Laboratory Standard Institute (CLSI). Structure elucidation of the isolated compounds was based primarily on 1D and 2D NMR analyses, including HMQC, HMBC, and NOESY correlations. Fractionation yielded three triterpenoids; (20S)-oxolupane-30-al, (20R)-oxolupane-30-al, and betulinic acid. The three compounds were active against Staphylococcus aureus check for this species in other resources ATCC 25923 and only (20S)-oxolupane-30-al against clinical isolate of Microsporum gypseum check for this species in other resources . The three compounds had no activity against Escherichia coli check for this species in other resources ATCC 25922, Enterococcus feacalis check for this species in other resources , Candida albicans check for this species in other resources ATCC 90028, Cryptococcus neoformans check for this species in other resources , Trichophyton mentagrophyte check for this species in other resources , Candida krusei check for this species in other resources , Microsporum gypseum check for this species in other resources , and Sacharomyces cerevisiae. These results explain and support the use of A. mellifera stem barks for the treatment of infectious diseases in traditional Kenya medicine.It also shows that the antimicrobial activity is concentrated in the triterpenoid fractions.

Keywords
Acacia mellifera; Antimicrobial pentacyclic triterpenes; Kenya medicinal plant.

 
© Copyright 2009 - African Journal Traditional, Complementary and Alternative Medicines
Alternative site location: http://journals.sfu.ca/africanem/index.php/ajtcam

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil