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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 6, No. 2, 2009, pp. 118 - 122
Bioline Code: tc09016
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 6, No. 2, 2009, pp. 118 - 122

 en Isolation And Characterization Of Antineoplastic Alkaloids From Catharanthus Roseus check for this species in other resources L. Don. Cultivated In Egypt
Shams, Khaled A.; Nazif, Naglaa M.; Abdel Azim, Nahla S.; Abdel Shafeek, KHaled A.; El-Missiry, Mostafa M.; Ismail, Shams I. & Seif El Nasr., Medhat M.

Abstract

Vinblastine and vincristine (the antileukemic agents) were isolated, in a pure form, from Catharanthus Roseus check for this species in other resources L. Don., cultivated in Egypt, by several chromatographic techniques. Five modified methods for the preparation of total alkaloids were carried out. All the isolated mixtures were evaluated by HPLC and HPTLC analyses. The antineoplastic alkaloids; vinblastine and vincristine, were isolated by the use of vacuum liquid chromatographic column on silica gel : aluminium oxide (1:1) mixed bed vacuum liquid chromatography (VLC), Charcoal column, and finally purified by centrifugally accelerated radial chromatography (Chromatotrone).

Keywords
Catharanthus roseus L., Apocyanaceae, Vinblastine, Vincrisitine, Antileuckemic alkaloids, VLC, HPLC, HPTLC.

 
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