Vinblastine and vincristine (the antileukemic agents) were isolated, in a pure form, from Catharanthus Roseus
L. Don., cultivated in Egypt, by several chromatographic techniques. Five modified methods for the
preparation of total alkaloids were carried out. All the isolated mixtures were evaluated by HPLC and HPTLC
analyses. The antineoplastic alkaloids; vinblastine and vincristine, were isolated by the use of vacuum liquid
chromatographic column on silica gel : aluminium oxide (1:1) mixed bed vacuum liquid chromatography (VLC),
Charcoal column, and finally purified by centrifugally accelerated radial chromatography (Chromatotrone).