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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 6, No. 2, 2009, pp. 186 - 194
Bioline Code: tc09024
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 6, No. 2, 2009, pp. 186 - 194

 en Antibacterial Activity Of Tabernaemontana Stapfiana check for this species in other resources Britten (Apocynaceae) Extracts
Ruttoh, Ernest K.; Tarus, Paul K.; Bii, Christine C.; Machocho, Alex K.; Karimi, Lucas K. & Okemo, Paul O.

Abstract

Antibacterial and phytochemical screening of methanolic, sequential extracts (hexane, dichloromethane, ethyl acetate and methanol) and alkaloid rich fractions of Tabernaemontana Stapfiana check for this species in other resources Britten was carried out. The phytochemical screening showed the presence of alkaloids, flavonoids, coumarins, tannins and saponins that have been associated with antimicrobial activity. The stem and root bark methanolic extracts showed good activity against the bacterial strains used including the multiple drug resistant Staphylococcus aureus check for this species in other resources strain with minimum inhibitory concentrations ranging from 15.6 to 500 mg/ml and minimum bactericidal concentrations ranging from 31.25 to 500 mg/ml. The sequential extracts of the root and stem bark had high antimicrobial activity with minimum inhibitory concentrations (MICs) ranging between 3.9 and 250 mg/ml and minimum bactericidal concentrations (MBCs) ranging between 7.8 and 500 mg/ml against the tested microorganisms. The dichloromethane extract of the alkaloid rich fractions however exhibited reduced antibacterial activities as compared to methanol and sequential extracts but the dichloromethane:methanol (4:1) mixture showed high activity with MICs ranging between 15.6 and 250 mg/ml. These antibacterial efficacy studies suggest that Tabernaemontana stapfiana check for this species in other resources Britten could be a source of antibacterial agents.

Keywords
Tabernaemontana stapfiana; Antibacterial; MICs; MBCs.

 
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