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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 6, No. 3, 2009, pp. 275 - 280
Bioline Code: tc09037
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 6, No. 3, 2009, pp. 275 - 280

 en Comparing The Cytotoxic Potential Of Withania somnifera check for this species in other resources Water And Methanol Extracts
Pretorius, Etheresia; Oberholzer, Hester Magdalena & Becker, Petrus Johannes

Abstract

The plant Withania somnifera check for this species in other resources (Linn.) (Solanacea) is a well-known herbal medicine used in many parts of the world. It has anti-inflammatory, antioxidant, and antitumor as well as neural protective properties. It seems as if the two most active withanolide components, namely withaferin A and withanolide D, found in methanol (MeOH) extracts, are responsible for the anti-inflammatory and antioxidant properties of the plant. The current research evaluated and compared the cytotoxic potential of water and methanol extracts of W. somnifera using a combined crystal violet MTT and Neutral Red assay. MRC-5 cells, a human embryonic lung-derived diploid fibroblast cell line, were the cells of choice. We found that the three lowest concentrations (0.007, 0.042, 0.250 μg/ml) of the plant material extracted in double distilled H2O and MeOH do not differ significantly in any of the assays. We therefore suggest that low concentrations of MeOH extracts (up to 0.250 μg/ml plant material) do not cause cell damage to the MRC-5 cells, however, higher levels should be avoided as cell viability and cell numbers are negatively influenced.

Keywords
Withania somnifera, anti-inflammatory; water extract; methanol extract

 
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