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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 7, No. 1, 2010, pp. 64-78
Bioline Code: tc10010
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 7, No. 1, 2010, pp. 64-78

 en Detection of antimicrobial compounds by bioautography of different extracts of leaves of selected South African tree species
Suleiman, M. M.; McGaw, L. J.; Naidoo, V. & Eloff, J. N.

Abstract

The hexane, acetone, dichloromethane and methanol extracts of Combretum vendae check for this species in other resources A.E. van Wyk (Combretaceae), Commiphora harveyi check for this species in other resources (Engl.) Engl. (Burseraceae), Khaya anthotheca check for this species in other resources (Welm.) C.DC (Meliaceae), Kirkia wilmsii check for this species in other resources Engl. (Kirkiaceae), Loxostylis alata check for this species in other resources A. Spreng. ex Rchb. (Anacardiaceae), Ochna natalitia check for this species in other resources (Meisn.) Walp. (Ochnaceae) and Protorhus longifolia check for this species in other resources (Bernh. Ex C. Krauss) Engl. (Anacardiaceae) were screened for their antimicrobial activity. The test organisms included bacteria ( Enterococcus faecalis check for this species in other resources , Escherichia coli check for this species in other resources , Pseudomonas aeruginosa check for this species in other resources and Staphylococcus aureus check for this species in other resources ), and fungi ( Aspergillus fumigatus check for this species in other resources , Candida albicans check for this species in other resources , Cryptococcus neoformans check for this species in other resources , Microsporum canis check for this species in other resources and Sporothrix schenckii check for this species in other resources ). A simple bioautographic procedure, involving spraying suspensions of the bacteria or fungi on thin layer chromatography (TLC) plates developed in solvents of varying polarities was used to detect the number of antibacterial and antifungal compounds present in the extracts. All the extracts had antimicrobial activity against at least one of the test microorganisms. This activity was denoted by white spots against a red-purple background on the TLC plates after spraying with tetrazolium violet. Twenty seven TLC plates; 9 for each solvent system and 3 different solvent systems per organism were tested in the bioautographic procedure. Of the bacteria tested, S. aureus was inhibited by the most compounds separated on the TLC plates from all the tested plants. Similarly, growth of the fungus C. neoformans was also inhibited by many compounds present in the extracts. Loxostylis alata appeared to be the plant extract with the highest number of inhibition bands when compared with other plants tested against both bacteria and fungi. This species was selected for in depth further study.

Keywords
Bioautography; Medicinal plants; Antifungal; Antibacterial; Synergism

 
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