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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 7, No. 2, 2010, pp. 98-103
Bioline Code: tc10015
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 7, No. 2, 2010, pp. 98-103

 en Antibacterial Activity Of Extracts Of Three Croton check for this species in other resources Species Collected In Mpumalanga Region In South Africa
Selowa, S.C.; Shai, L.J.; Masoko, P.; Mokgotho, M.P. & Magano, S.R.

Abstract

The antibacterial activities of three Croton check for this species in other resources species were compared using bioautography and the serial microdilution methods. The methanolic extracts of all the species had low activity against Escherichia coli check for this species in other resources . The highest activity was observed with Croton megalobotrys check for this species in other resources against Enterococcus faecalis with a minimal inhibitory concentration (MIC) value of 0.02 mg/ml. Croton steenkapianus check for this species in other resources extracts were the least active of the species investigated, only managing an MIC value of 0.625 mg/ml against Pseudomonas aeruginosa. Croton megalobotrys leaf powder was serially extracted using solvents of various polarities. The lowest MIC value (0.06 mg/ml) of the serially extracted fractions was observed with acetone against Pseudomonas aeruginosa. The liquid-liquid fractions of the methanol extract of Croton megalobotrys were also tested. The lowest MIC value of 0.02 mg/ml was observed with n-hexane fraction against Enterococcus faecalis. The carbon tetrachloride fraction was further fractionated using column chromatography with silica as the immobile phase. The resulting seven fractions were tested for activity following the bioassay-guided practice, and it emerged that the first three fractions had active compounds against Staphylococcus aureus when the bioautography method was used.

Keywords
Croton megalobotrys; C. steenkapianus; C. silvaticus; antibacterial activity.

 
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Alternative site location: http://journals.sfu.ca/africanem/index.php/ajtcam

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