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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 8, No. 5s, 2011, pp. 33-45
Bioline Code: tc11067
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 8, No. 5s, 2011, pp. 33-45

 en Analysis of the Extracts of Isatis tinctoria check for this species in other resources by New Analytical Approaches of HPLC, MS and NMR
Zhou, Jue & Qu, Fan

Abstract

The methods of extraction, separation and analysis of alkaloids and indole glucosinolates (GLs) of Isatis tinctoria check for this species in other resources were reviewed. Different analytical approaches such as High-pressure Liquid Chromatography (HPLC), Liquid Chromatography with Electrospray Ionization Mass Spectrometry (LC/ESI/MS), Electrospray Ionization Time-Of-Flight Mass Spectrometry (ESI-TOF-MS), and Nuclear Magnetic Resonance (NMR) were used to validate and identity of these constituents. These methods provide rapid separation, identification and quantitative measurements of alkaloids and GLs of Isatis tinctoria. By connection with different detectors to HPLC such as PDA, ELSD, ESI- and APCI-MS in positive and negative ion modes, complicated compounds could be detected with at least two independent detection modes. The molecular formula can be derived in a second step of ESI-TOF-MS data. But for some constituents, UV and MS cannot provide sufficient structure identification. After peak purification, NMR by semi-preparative HPLC can be used as a complementary method.

Keywords
Isatis tinctoria; HPLC; MS; NMR; indole glucosinolates (GLs); alkaloids

 
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