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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 9, No. 1, 2012, pp. 88-93
Bioline Code: tc12013
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 9, No. 1, 2012, pp. 88-93

 en BIOACTIVE DITERPENES AND SESQUITERPENES FROM THE RHIZOMES OF WILD GINGER ( SIPHONOCHILUS AETHIOPICUS check for this species in other resources (SCHWEINF) B.L BURTT)
Igoli, Ngozichukwuka Peace; Obanu, Zak Ahamefula; Gray, Alexander I. & Clements, Carol

Abstract

Wild ginger ( Siphonochilus aethiopicus check for this species in other resources (Schweinf) B.L Burtt) is used in traditional medicines in the West and South of Africa. In the present study, the crude hexane extract of wild ginger was evaluated for in vitro bioactivity. The components isolated from the plant for the first time are: epi-curzerenone, furanodienone (sesquiterpenes), 8(17),12E-labdadiene-15,16-dial, 15-hydroxy-8(17),12E-labdadiene-16-al and 16-oxo-8(17),12E-labdadiene-15-oic acid (labdanes). Cytotoxicity determinations using five cell lines: SH-SY5Y (human, Caucasian, bone marrow, neuroblastoma), Jurkat (human, peripheral blood, leukaemia T cell), L929 (mouse, CH3/connective tissue, areolar and adipose tumour cells), Hep G2 (human, Caucasian, hepatocellular carcinoma) and Hs 27 (normal, human, foreskin cells) were carried out. Anti-trypanosomal activity against Trypanosoma brucei brucei check for this species in other resources (S427) blood stream forms and anti-bacterial activity against Mycobacterium aurum check for this species in other resources (CIP .104482) were also investigated. Activity against M. aurum was moderate and at 100μg/ml, the crude extract together with the labdanes showed specific cytotoxicity, indicating anti-cancer potency. Anti-trypanosomal activity was observed in the crude extract which increased with the pure components: 8(17),12E-labdadiene-15,16-dial (MIC = 5.3 μM) and the sesquiterpenoids (MIC = 6.9 μM) as compared to suramin activity (MIC = 10 μM). This anti-trypanosomal activity which is being reported for the first time indicates possible usage against sleeping sickness and nagana in cattle.

Keywords
Anti-bacterial activity; Anti-trypanosomal activity; Cytotoxicity; Labdane diterpenoids; Sesquiterpenoids; Siphonochilus aethiopicus

 
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