This study evaluated the antioxidant and anti-fatigue activities of flavonoids from Puerariae radix
(FPR). In vitro
antioxidant activities of FPR were investigated through hydroxyl and superoxide radical scavenging activities. In vivo
activity of FPR was investigated through loaded swimming exercise of mice. Results showed that FPR had not only in vitro
antioxidant activities, but also an in vivo
anti-fatigue activity in mice. FPR possessed superoxide and hydroxyl radical scavenging
activity in in vitro
experimental studies. In vivo experimental studies, FPR could evidently extend exhaustive swimming time of
mice, inhibit the increase of blood lactic acid (BLA), decrease serum urea nitrogen (BUN) and malondialdehyde (MDA) contents,
promote increases in the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPX) of mice after swimming.
The results provided an important basis for developing the FPR as a novel antioxidant and anti-fatigue compound.