Juglans mandshurica
Maxim is a traditional herbal medicines in China, and its anti-tumor bioactivities are of
research interest. Bioassay-guided fractionation method was employed to isolate anti-tumor compounds from the stem barks of
the
Juglans mandshurica Maxim. The anti-tumor effect and biological activities of the extracted compound JMM6 were
studied in BEL-7402 cells by MTT, Cell cycle analysis, Hoechst 33342 staining, Annexin V-FITC/PI assay and Detection of
mitochondrial membrane potential (△Ψm). After treatment with the JMM6, the growth of BEL-7402 cells was inhibited and
cells displayed typical morphological apoptotic characteristics. Further investigations revealed that treatment with JMM6
mainly caused G2/M cell cycle arrest and induced apoptosis in BEL-7402 cells. To evaluate the alteration of mitochondria in
JMM6 induced apoptosis. The data showed that JMM6 decreased significantly the △Ψm, causing the depolarization of the
mitochondrial membrane. Our results show that the JMM6 will have a potential advantage of anti-tumor, less harmful to
normal cells. This paper not only summarized the JMM6 pick-up technology from
Juglans mandshurica Maxim and
biological characteristic, but also may provide further evidence to exploit the potential medicine compounds from the
stem-barks of the Chinese
Juglans mandshurica Maxim.
