The effects of
Duranta repens
fruits were investigated on H
2O
2 induced oxidative cell death to evaluate its antioxidative potential
in vitro. HEK293T cells were treated with different concentrations [0-1000 μg/ ml] of ethanol extract (E-Ex) and methanol extract (M-Ex) of
D. repens for 24h, and then treated with 100 μM H
2O
2 for 24h. Cell viability, antioxidant parameters of cells, and antioxidant constituents of the extracts were determined. Treatment with limited dose of E-Ex or M-Ex increased the survival rate of H
2O
2–treated HEK293T cells, however the extra-high dose showed growth inhibitory effect. Treatment with E-Ex or M-Ex protected cellular lipid per-oxidation.
In vitro analyses showed the 2,2-diphenyl-1-picrylhydrazyl and H
2O
2 scavenging activities as well as reducing potential of the extracts. We report here that the limited dose of E-Ex and M-Ex possess antioxidative potential, which can protect H
2O
2-induced oxidative cell damage.