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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 10, No. 5, 2013, pp. 352-355
Bioline Code: tc13128
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 10, No. 5, 2013, pp. 352-355

 en EFFECTS OF ETHANOL EXTRACT OF RADIX SOPHORAE FLAVESCENTIS check for this species in other resources ON ACTIVITY OF COLON CANCER HT29 CELLS
XIAO, Zhi-Ming; WANG, Ai-Min; WANG, Xiao-Yan & SHEN, Shou-Rong

Abstract

This paper mainly studied the inhibitory effect of total ethanol extract of Radix Sophorae Flavescentis check for this species in other resources on proliferation of colon cancer HT29 cells. By reflux extraction method and with ethanol as extraction solvent, different extracts were obtained at different ethanol concentrations, different solid-liquid ratios, and at different times. And their inhibitory activities against HT29 cells were compared using MTT assay. The experimental results showed that the extraction processes under three conditions can all draw relatively high inhibition rates. The optimum ethanol extraction process conditions were as follows: a solid-liquid ratio of 1:9, 80 min of heat reflux extraction with 95% ethanol.

Keywords
Sophora flavescens; cancer HT29; Orthogonal experiment,; inhibition

 
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