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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 10, No. 6, 2013, pp. 464-468
Bioline Code: tc13150
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 10, No. 6, 2013, pp. 464-468

 en A STUDY ON SCREENING OF OSTEOSARCOMA U2OS CELL INHIBITING ACTIVE COMPONENTS FROM NIDUS VESPAE check for this species in other resources
Jiang, Zhenhuan; Jiang, Jiannong; Wang, Qiang; Zhang, Leiyan; Wu, Chenguang & Yang, Huilin

Abstract

This paper mainly examined the anti-osteosarcoma activity of total flavonoids extract from traditional Chinese medicine, nidus vespae check for this species in other resources . Orthogonal design was used to design the extraction process of total flavonoids. L(34) orthogonal test was performed and the extracts obtained by three optimal extraction processes were used for anti-tumour activity screening in order to determine the optimal anti-tumour effective component of nidus vespae. MTT assay was used to investigate the effect of nidus vespae extract on proliferation activity of osteosarcoma cells. Meanwhile, U2OS cell inhibitory capacities of extracts in three groups with higher total flavonoid contents were investigated and compared, and inhibition rates were calculated.The results showed that the optimal extraction process was ethanol concentration of 95%, 12-fold amount of ethanol relative to the weight of medicinal material, extraction times of 3 times, and extraction time of 2 hours. 9 extraction processes all showed proportional trend of cancer cell inhibition rate to extract concentration.

Keywords
Nidus vespae; U2OS; Inhibiting Actuve; MTT

 
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