African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
Vol. 11, No. 1, 2014, pp. 62-66
Bioline Code: tc14008
Full paper language: English
Document type: Research Article
Document available free of charge
African Journal of Traditional, Complementary and Alternative Medicines, Vol. 11, No. 1, 2014, pp. 62-66
© Copyright 2014 - African Journal of Traditional, Complementary and Alternative Medicines
EFFECTS OF VIETNAMESE SOPHORA ROOT ON GROWTH, ADHESION, INVASION AND MOTILITY OF MELANOMA CELLS|
Zhang, Dongxing; Liu, Shi; Yao, Mingzhu; Liu, Xiong & Lai, Hongtao
Background: Vietnamese Sophora Root mainly contains active constituents such as alkaloids, and it has anti-tumour, antibacterial, and
anti-inflammatory effects. The objective of the paper was to study the effects of Vietnamese Sophora Root on growth, adhesion, invasion and
motility of mouse melanoma B16BL6 cells, and to preliminarily explore its mechanism of action.
Materials and Methods: MTT assay was used to detect the effect of Vietnamese Sophora Root aqueous extract on B16BL6 cell proliferation. Cell
adhesion assay, reconstituted basement membrane invasion assay and chemotactic motility assay were used to observe the effects of Vietnamese
Sophora Root aqueous extract on adhesion, invasion and motility of B16BL6 cells.
Results: Different concentrations of Vietnamese Sophora Root aqueous extracts had different degrees of inhibitory effects on B16BL6 proliferation.
With the decrease of concentration, the proliferation inhibitory effect decreased and even turned to promoting effect. The extract significantly
inhibited the adhesion of B16BL6 cells to the basement membrane component LN, and had a significant effect on both the invasive and migratory
capacities of B16BL6 cells through the basement membrane.
Conclusion: We concluded that the aqueous extract of Vietnamese Sophora Root can inhibit the proliferation of melanoma cells, as well as their
adhesion and movement.
Vietnamese Sophora Root; B16BL6; adhesion; invasion
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