Background: Leucaena leucocephala
is native to Southern Mexico and Northern Central America, but is now naturalized throughout the tropics. The
phyto-chemical data of L. leucocephala
revealed the presence of terpenes, flavonoids, coumarins and sterols. Various parts of L. leucocephala
been reported to have medicinal properties.
Materials and Methods:
Flavonoids were isolated from the aerial parts of L. leucocephala
. Antioxidant activity of the extracts and the isolated
compounds was evaluated using (DPPH), as well as their cytotoxic activity using a single tumor [Ehrlish ascites carcinoma cells].
The flavonoidal constituents isolated from chloroform, ethyl acetate and n-butanol fractions of the aqueous alcoholic extract of aerial parts of
were identified as Caffeic acid, Isorhamnetin, Chrysoeriol, Isorhamnetin 3-O-galactoside, Kaempferol-3-O-rubinoside,
Quercetin-3-O-rhamnoside and Luteolin-7-glucoside. Chemical structures of the isolated compounds were identified by TLC, PC and spectral
techniques (UV, 1
H -NMR and MS). The ethyl acetate fraction and the isolated flavonoidal compounds showed high antioxidant activity compared to
Trolox (standard antioxidant compound). The different fractions and isolated compounds of Leucaena leucocephala
exhibited no cytotoxic activity
against Ehrlich- ascitis carcinoma cell line at the tested concentrations.
This is the first record of the flavonoids in the aerial parts of Leucaena leucocephala
(L.) except Quercetin-3-O-rhamnoside.